|

Step 1: Open a TINKER coordinate file. For example "tinker/test/enkephalin.xyz". |

Step 2: Specify keywords that control aspects of the
modeling commands. The default keywords for enkephalin, stored in the
"tinker/test/enkephalin.key" file, were loaded automatically when the enkephalin coordinate file was opened. Using this set of keywords is a good
place to begin. Notice that enkephalin uses the MM3PRO parameter set. Simply
changing the "PARAMETER" keyword to a different force field parameter set
does not change the atom type identifiers in the coordinate file. |
|

Step 3: Execute a modeling command.
The "NEWTON" minimizer is quite
fast for smaller systems (where storage of the Hessian is not an issue) so it is a good choice for optimizing enkephalin to
a local minimum. For protein size systems "MINIMIZE", which only uses first
derivatives, is a safer choice. |

Step 4: Observe structural and/or energetic results of the
modeling command. The Newton
optimization finds a local minimum for enkephalin after 60 iterations - less
than a minute of CPU time on current hardware. TINKER creates a new
structure file, "tinker/test/enkephalin.xyz_2", to store the local minimum.
Force Field Explorer monitors the calculation, interactively updating
coordinates, and loads enkephalin.xyz_2 over the top of enkephalin.xyz when
TINKER completes. |
|

Step 5: In a "real" molecular engineering setting,
hopefully the investigator has learned something from the modeling command.
They might then change a few keywords and/or execute another modeling
command. In the future we hope to add editing features to close the design
loop. In this case, try removing the implicit solvation model (set it to
"absent") from the Keyword Editor and re-optimizing the structure. |